PPT Slide

FL-mab

+

PE-mab

+

TC-mab

50 µl

washed, and

blocked*

whole blood or

bone marrow

15 min. on ice

lyse,

centrifuge,

decant,

blot,

and

resuspend

pellet

wash,

fix,

and

analyse

THREE COLOR SOP

*add 10 µl mIg (10 mg/ml) to 1 ml washed whole blood.

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