PPT Slide

THREE COLOR IMMUNOFLUORESCENCE

1. Ig Block + MAB •

B- second antibody F(ab')

2

• Ig Block + FL- MAB + PE-MAB + TC- Avidin •

2. Ig Block + FL-MAB + PE-MAB + B-MAB •

TC-Avidin •

3. Ig Block + FL-MAB + PE-MAB + TC-MAB •

TC(third color) = PE/TR or PE/CY5 tandem or PerCP

Previous slide

Next slide

Back to first slide

View graphic version